| 货号 | MAB6795-SP |
| 别名 | FLJ12795; FLJ13221; FLJ25756; IGDCC5; immunoglobulin superfamily, DCC subclass, member 5; Protein Shen-Dan; protogenin homolog (Gallus gallus); protogenin; PRTG; RPTG; Shen-Dan |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human Protogenin in direct ELISAs. |
| 使用方法 | Neutralization: Measured by its ability to neutralize Protogenin-induced adhesion in the P19 mouse embryonal carcinoma cell line. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 10 µg/mL Recombinant Human Protogenin. |
| 来源 | Monoclonal Mouse IgG1 Clone # 814403 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 283659 (Human); 235472 (Mouse); 315806 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Protogenin Phe36-Ala952 Accession # Q2VWP7 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | PRTG (Protogenin; also Protein Shen-Dan) is a 175-180 kDa transmembrane (TM) member of the DEAL (DCC et al) family of proteins. It is expressed in the embryo in the period before E10.5 in mouse (day 28 in human), and appears in a widespread distribution. Through homophilic intercellular interaction, it regulates initial epiblast cell migration/ingression, thus impacting somite organization. Via its actions as a neural tube epithelial cell receptor, it binds soluble 60 kDa ERdj3, resulting in a suppression of neuron differentiation. Mature human PRTG is an 1115 amino acid (aa) type I TM glycoprotein. It contains a 917 aa extracellular domain (ECD) (aa 36-952) and a 180 aa cytoplasmic region. The ECD possesses four consecutive Ig-like domains (aa 36-411) followed by five FN type III domains (aa 419-914). There is one potential splice variant that shows a 49 aa substitution for Ala325 coupled to a deletion of aa 516-541. The ECD of human PRTG shares 93% aa identity with the ECD of mouse PRTG. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Adhesion Induced by Protogenin and Neutralization by Human Protogenin Antibody. Recombinant Human Protogenin (Catalog # 6795-PR) supports the adhesion in the P19 mouse embryonal carcinoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Protogenin (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Protogenin Monoclonal Antibody (Catalog # MAB6795). The ND50 is typically 0.4-2.4 µg/mL. |
