Human VEGF-D MAb (Clone 78923) (25 UG)【科瑞奥博】

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Human VEGF-D MAb (Clone 78923) (25 UG)

货号： MAB286-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB286-SP
别名	c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGF-DVEGFDc-Fos-induced growth factor
全称	Vascular Endothelial Growth Factor D/cFos-induced Growth Factor
反应种属	Human
应用	Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human VEGF-D in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) VEGF‑D, rmVEGF-B, recombinant human (rh) VEGF-B and rhVEGF is observed.
使用方法	Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize VEGF‑D-induced proliferation in HMVEC human microvascular endothelial cells. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548. The Neutralization Dose (ND₅₀) is typically 3‑8 µg/mL in the presence of 1 µg/mL Recombinant Human VEGF‑D.
来源	Monoclonal Mouse IgG₁ Clone # 78923
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2277 (Human); 14205 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Primary Tumor-Secreted Lymphangiogenic Factors Induce Pre-Metastatic Lymphvascular Niche Formation at Sentinel Lymph Nodes in Oral Squamous Cell Carcinoma. Authors: Wakisaka N, Hasegawa Y, Yoshimoto S, Miura K, Shiotani A, Yokoyama J, Sugasawa M, Moriyama-Kita M, Endo K, Yoshizaki T PLoS ONE, 2015;10(12):e0144056. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Tbx1 regulates Vegfr3 and is required for lymphatic vessel development. Authors: Chen L, Mupo A, Huynh T, Cioffi S, Woods M, Jin C, McKeehan W, Thompson-Snipes L, Baldini A, Illingworth E J. Cell Biol., 2010;189(3):417-24. Species: Human Sample Type: Whole Cells Application: ICC VEGF-DdeltaNdeltaC mediated angiogenesis in skeletal muscles of diabetic WHHL rabbits. Authors: Roy H, Bhardwaj S, Babu M, Lahteenvuo JE, Yla-Herttuala S Eur. J. Clin. Invest., 2010;40(5):422-32. Species: Rabbit Sample Type: Whole Tissue Application: IHC Paraffin-embedded Novel vascular endothelial growth factor D variants with increased biological activity. Authors: Toivanen PI, Nieminen T, Viitanen L, Alitalo A, Roschier M, Jauhiainen S, Markkanen JE, Laitinen OH, Airenne TT, Salminen TA, Johnson MS, Airenne KJ, Yla-Herttuala S J. Biol. Chem., 2009;284(23):16037-48. Species: Human Sample Type: Cell Culture Supernates Application: WB Prognostic significance of vascular endothelial growth factor D in breast carcinoma with long-term follow-up. Authors: Nakamura Y, Yasuoka H, Tsujimoto M, Yang Q, Imabun S, Nakahara M, Nakao K, Nakamura M, Mori I, Kakudo K Clin. Cancer Res., 2003;9(2):716-21. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome. Authors: Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ Am. J. Pathol., 2002;160(4):1405-23. Species: Human Sample Type: Whole Tissue Application: IHC Fresh/Frozen
纯化方式	Protein A or G purified from ascites
免疫原	Mouse myeloma cell line NS0-derived recombinant human VEGF-D Phe93-Ser201 Accession # O43915
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Vascular endothelial growth factor D (VEGF-D), also known asc-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cystine knot structure (1-3). VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C‑terminal propeptide extensions around the VEGF homology domain (VHD) (1, 2). Proteolytic processing of the 354 aa VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non‑covalently linked 42 kDa homodimers of the 117 aa VHD (4-6). Mature human VEGF-D shares 94%, 95%, 99%, 97%, and 93% aa identity with mouse, rat, equine, canine, and bovine VEGF-D, respectively (4, 5). It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin (1-4). Mouse and human VEGF-D are ligands for VEGF Receptor 3 (VEGF R3, also called Flt-4) that are active across species and show enhanced affinity when processed (7). Processed human VEGF-D is also a ligand for VEGF R2, also called Flk-1 or KDR (7). VEGF R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium (1, 2). While VEGF-C is the critical ligand for VEGF R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth (8-10). Both promote tumor lymphangiogenesis (11). Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF R3 signaling in lymphangiogenesis, while binding to integrin alpha 9 beta 1 mediates endothelial cell adhesion and migration (12, 13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Roy, H. et al. (2006) FEBS Lett. 580:2879. Otrock, Z.H. et al. (2007) Blood Cells Mol. Dis. 38:258. Yamada, Y. et al. (1997) Genomics 42:483. Stacker, S.A. et al. (1999) J. Biol. Chem. 274:32127. McColl, B.K. et al. (2003) J. Exp. Med. 198:863. McColl, B.K. et al. (2007) FASEB J. 21:1088. Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:19166. Baldwin, M.E. et al. (2005) Mol. Cell. Biol. 25:2441. Karpanen, T. et al. (2006) Am. J. Pathol. 169:708. Orlandini, M. et al. (2006) J. Biol. Chem. 281:17961. Stacker, S.A. et al. (2001) Nature Med. 7:186. Karpanen, T. et al. (2006) FASEB J. 20:1462. Vlahakis, N.E. et al. (2005) J. Biol. Chem. 280:4544.

参考图片

Cell Proliferation Induced by VEGF‑D and Neutralization by Human VEGF‑D Antibody.

Recombinant Human VEGF‑D (Catalog # 622-VD) stimulates proliferation in HMVEC human microvascular endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF‑D (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGF-D Monoclonal Antibody (Catalog # MAB286). The ND₅₀ is typically 3-8 µg/mL.

VEGF‑D in Human Placenta. VEGF‑D was detected in immersion fixed frozen sections of human placenta (cross-section through a blood vessel) using 15 µg/mL Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB286) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.