Mouse GDF-9 MAb (Clone 785123) (25 UG)【科瑞奥博】

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Mouse GDF-9 MAb (Clone 785123) (25 UG)

货号： MAB739-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB739-SP
别名	GDF-9; growth differentiation factor 9; growth/differentiation factor 9
全称	Growth Differentiation Factor 9
应用	Neutralization
目标/特异性	Detects mouse GDF-9 in direct ELISAs.
使用方法	Neutralization: Measured by its ability to neutralize GDF‑9-induced growth inhibition in the Mv1Lu mink lung epithelial cell line. The Neutralization Dose (ND₅₀) is typically 0.4-2.4 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse GDF‑9.
来源	Monoclonal Rat IgG_2A Clone # 785123
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2661 (Human); 14566 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant mouse GDF-9 Met1-Arg441 Accession # Q07105
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Growth differentiation factor-9 (GDF-9) is a member of the transforming growth factor-beta (TGF-beta ) superfamily, and is an oocyte secreted paracrine factor essential for mammalian ovarian folliculogenesis (1‑2). Mouse GDF-9 is synthesized as a 441 amino acid (aa) prepropeptide that contains a 29 aa signal sequence, a 277 aa propeptide, and a 135 aa mature chain. Residues 340‑441 constitute a TGF-beta like domain. In addition, there is one potential site of N-linked glycosylation in the mature chain. Unlike other members of the TGF-beta superfamily, GDF-9 lacks the conserved cysteine residue that is believed to form the sole disulfide linkage between subunits in other family members (3). Mature mouse GDF-9 shares 90% aa sequence identity with mature human GDF 9. The protein is expressed throughout the development of the maturing follicle (2). GDF-9 functions as a paracrine factor in the regulation of granulosa cell proliferation and differentiation, and is essential for fertility (2, 4). Studies on GDF-9 null mice have demonstrated arrested follicular development at the primary follicle stage (5). Mouse GDF-9 induces Smad2 phosphorylation and inhibin production in rat diethylstilbestrol treated granulosa cells (6) and in human granulosa-luteal cells (7). The downstream signaling actions of GDF 9 are mediated by the type I receptor, activin receptor-like kinase 5 (ALK5), initiating the subsequent activation of Smad2 and Smad3 (2, 8). GDF 9 uses the BMP type II receptor (BMPRII) as its other signaling receptor (2, 9).
运输条件	Blue Ice
存放说明	4℃
参考文献	McGrath, S.A. et al. (1995) Mol. Endocrinol. 9:131. Mottershead, D.G. et al. (2008) Mol. Cell. Endocrinol. 283:58. McPherron, A.C. and S.-J. Lee (1992) J. Biol. Chem. 268:3444. Gilchrist, R.B. et al. (2006) J. Cell. Sci. 119:3811. Dong, J. et al. (1996) Nature 383:531. Roh, J.S. et al. (2003) Endocrinology 144:172. Kaivo-Oja, N. et al. (2003) J. Clin. Endocrinol. Metab. 88:755. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653. Vitt, U.A. et al. (2002) Biol. Reprod. 67:473.

参考图片

Growth Inhibition Induced by GDF‑9 and Neutralization by Mouse GDF‑9 Antibody. Recombinant Mouse GDF‑9 (Catalog # 739-G9) induces growth inhibition in the Mv1Lu mink lung epithelial cell line in the presence of in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Growth Inhibition elicited by Recombinant Mouse GDF‑9 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GDF‑9 Monoclonal Antibody (Catalog # MAB739). The ND₅₀ is typically 0.4-2.4 µg/mL.