Mouse BMP-5 MAb (Clone 781321) (25 UG)【科瑞奥博】

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Mouse BMP-5 MAb (Clone 781321) (25 UG)

货号： MAB6176-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB6176-SP
别名	BMP-5; bone morphogenetic protein 5; MGC34244
全称	Bone Morphogenetic Protein 5
反应种属	Mouse
应用	Neutralization
目标/特异性	Detects mouse BMP-5 in ELISA.
使用方法	Neutralization: Measured by its ability to neutralize BMP‑5-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Asahina, I. et al. (1996) Exp. Cell Res. 222:38.The Neutralization Dose (ND₅₀) is typically 5-25 μg/mL in the presence of 2 μg/mL Recombinant Mouse BMP‑5.
来源	Monoclonal Rat IgG₁ Clone # 781321
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	653 (Human); 12160 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant mouse BMP-5 Ala315-His452 Accession # P49003
生物活性	Mouse
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	Bone Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 452 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 20 kDa C-terminal mature protein (2, 3). Mature BMP-5 contains seven conserved cysteine residues involved in formation of the cysteine knot and the single interchain disulfide bond. Biologically active BMP-5 is a disulfide-linked homodimer of the C-terminal mature protein. Mature mouse BMP-5 shares 96% and 99% aa sequence identity with human and rat BMP-5, respectively. Cellular responses to BMP-5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1). BMP-5 is expressed by chondrocytes in proliferating and hypertrophic zones of bone growth plates (4). It contributes to limb development by promoting proliferation and differentiation of chondrocytes as well as apoptosis of undifferentiated mesoderm (4, 5). Genetic defects in BMP-5 which cause C-terminal truncation or loss of the proteolytic cleavage site result in multiple skeletal abnormalities, including the short ear phenotype in mice (3, 6). BMP-5 is also expressed by ovarian granulosa cells where it functions as an autocrine factor to promote GC proliferation and inhibit their production of progesterone (7). In the nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic neuronal differentiation (8, 9). It is upregulated in oral squamous carcinoma cells and induces the apoptosis of some myeloma cell lines (10, 11).
运输条件	Blue Ice
存放说明	4℃
参考文献	Chen, D. et al. (2004) Growth Factors 22:233. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843. King, J.A. et al. (1994) Dev. Biol. 166:112. Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56. Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39. Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35. Pierre, A. et al. (2005) Biol. Reprod. 73:1102. Beck, H.N. et al. (2001) BMC Neurosci. 2:12. Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367. Jin, Y. et al. (2001) Oral Oncol. 37:225. Ro, T.B. et al. (2004) Oncogene 23:3024.

参考图片

Alkaline Phosphatase Production Induced by BMP‑5 and Neutralization by Mouse BMP‑5 Antibody. Recombinant Mouse BMP‑5 (Catalog # 6176-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse BMP‑5 (2 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse BMP‑5 Monoclonal Antibody (Catalog # MAB6176). The ND₅₀ is typically 5-25 μg/mL.