| 货号 | MAB7307-SP |
| 别名 | BIT; BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; CD172a antigen; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MFRtyrosine phosphatase SHP substrate 1; MyD-1 antigen; MYD1; MYD-1; P84; protein tyrosine phosphatase, non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRPA; SIRPalpha; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3; SIRPtyrosine-protein phosphatase non-receptor type substrate 1 | 全称 | Signal-regulatory Protein alpha |
| 反应种属 | Rat |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects rat SIRP-alpha /CD172a in direct ELISA and Western Blot. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Neutralization: Measured by its ability to neutralize SIRP alpha /CD172a-induced adhesion in mouse red blood cells. The Neutralization Dose (ND50) is typically 0.04-0.16 μg/mL in the presence of 6 μg/mL Recombinant Rat SIRP alpha /CD172a. |
| 来源 | Monoclonal Mouse IgG2B Clone # 772734 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 140885 (Human); 19261 (Mouse); 25528 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat SIRP-alpha /CD172a Lys32-Asn373 Accession # P97710 |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | SIRP alpha (Signal regulatory protein alpha; also CD172a, Shps1 and Bit) is a variably glycosylated, 85-120 kDa member of the SIRP ‘family of proteins. It is expressed on neurons, macrophages, monocytes, granulocytes and dendritic cells. SIRP alpha is phosphorylated/activated in response to cell adhesion. This may, or may not, involve binding to known ligands CD47, SP-A and SP-D. Following phosphorylation, SIRP alpha binds to SHP-1 and -2, resulting in the negative regulation of immune system activity. Mature rat SIRP alpha is a 478 amino acid (aa) type I transmembrane glycoprotein. It contains a 342 aa extracellular region (aa 32-373) that possesses one V-type and two C1-type Ig-like domains. Its cytoplasmic domain possesses two ITIMs that interact with protein phosphatases. There is one potential splice variant that shows a four aa insertion after Gln424. Over aa 32-373, rat SIRP alpha shares 63% and 73% aa sequence identity with human and mouse SIRP alpha, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Rat SIRP alpha /CD172a by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line and rat brain tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Rat SIRP alpha /CD172a Monoclonal Antibody (Catalog # MAB7307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for SIRP alpha /CD172a at approximately 75-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Adhesion Induced by SIRP alpha / CD172a and Neutralization by Rat SIRP alpha /CD172a Antibody. Recombinant Rat SIRP alpha /CD172a (Catalog # 7307-SA) supports the adhesion in mouse red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Rat SIRP alpha /CD172a (6 μg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat SIRP alpha /CD172a Monoclonal Antibody (Catalog # MAB7307). The ND50 is typically 0.04-0.16 μg/mL. | |
Detection of SIRP alpha /CD172a in PC‑12 Rat Cell Line by Flow Cytometry. PC‑12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat SIRP alpha /CD172a Monoclonal Antibody (Catalog # MAB7307, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). |
