| 货号 | MAB620-SP |
| 别名 | HHV5wtgp117; vCXC1; vCXCL1; Y18 | 全称 | Cytomegalovirus UL146 |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects viral CMV UL146/vCXC1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant humanCXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12,recombinant rat CXCL1, 3, recombinant viral MIP-I, -II, or -III is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CMV UL146/vCXC1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1.5-7.5 µg/mL in the presence of 0.3 µg/mL Recombinant Viral CMV UL146/vCXC1. |
| 来源 | Monoclonal Mouse IgG2A Clone # 76520 |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant viral CMV UL146/vCXC1 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Viral |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Cytomegalovirus (CMV), a member of the beta herpesvirus subfamily, typically causes subclinical or latent infections in the normal adult population. However, CMV can cause congenital disease during pregnancy and is a human opportunistic pathogen that affects immunocompromised individuals. The CMV genome has been shown to contain homologs of cellular immunomodulatory proteins, including US28 (a CC chemokine receptor) and a MHC class I homolog. Virulent CMV clinical isolates have also been shown to carry at least 19 genes, designated UL133-UL151, that are not found in laboratory strains that have lost virulence characteristics. Two of these genes, UL146 and UL147, exhibit sequence similarity to CXC chemokines. The CMV UL146 open-reading frame encodes a 117 amino acid residue precursor protein with a predicted 22 residues signal peptide that is cleaved to generate the mature protein. Recombinant UL146 has been shown to induce calcium mobilization, chemotaxis and degranulation of neutrophils. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Chemotaxis Induced by CMV UL146/vCXC1 and Neutralization by CMV UL146/vCXC1 Antibody. Recombinant Viral CMV UL146/vCXC1 (Catalog # 620-CM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Viral CMV UL146/vCXC1 (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Viral CMV UL146/vCXC1 Monoclonal Antibody (Catalog # MAB620). The ND50 is typically 1.5‑7.5 µg/mL. |
