Canine IL-17A MAb (Clone 665909) (25 UG)【科瑞奥博】

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Canine IL-17A MAb (Clone 665909) (25 UG)

货号： MAB5848-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB5848-SP
别名	CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
全称	Interleukin 17
反应种属	Canine
应用	Neutralization
目标/特异性	Detects canine IL-17 in direct ELISAs. In direct ELISAs, approximately 25%cross-reactivity with recombinant human (rh) IL-17A is observed and nocross-reactivity with rhIL-17F or recombinant mouse IL-17A is observed.
使用方法	Neutralization: Measured by its ability to neutralize IL‑17-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND₅₀) is typically 5-30 ng/mL in the presence of 5 ng/mL Recombinant Canine IL‑17.
来源	Monoclonal Mouse IgG_2A Clone # 665909
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant canine IL-17 Gly26-Ala155 Accession # NP_001159350
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Canine
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.5 mg/mL.
背景	Interleukin 17 (IL-17; also IL-17A and CTLA-8) is a 17 kDa member of the IL-17 family of cytokines (1). Members of this family demonstrate a structural motif termed a cysteine knot which characterize a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (2-4). Based on the amino acid (aa) sequence alignment with human IL-17, canine IL-17 is 130 aa in length. It is secreted as a 35 kDa disulfide-linked homodimer and as a 40 kDa disulfide-linked heterodimer with IL-17F (5). Canine IL‑17 is 81% identical on the aa level to human IL-17. IL-23 drives Th17 lymphocytes to produce IL-17 (6-8). IL-17’s production has also been demonstrated in gamma δ T cells (9), CD8⁺ memory T cells (10-11), eosinophils (12), neutrophils (10), and monocytes (13). Studies have identified that the widely expressed receptors IL‑17RA and IL-17RC form a heterodimer for the binding of IL-17 (6, 14-15). The predominant function of IL-17 is thought to be as a proinflammatory mediator through a variety of mechanisms (16). Locally, IL‑17 stimulates production of IL-6, prostaglandin E and nitric oxide (16-19), and synergy with other inflammatory cytokines such as TNF-alpha, IL‑1 beta and IFN -gamma leads to up-regulation of gene expression and progression and amplification of local inflammation (16, 20-22). IL‑17 also mediates chemotaxis of neutrophils and monocytes to sites of inflammation through the chemoattractant mediators IL-8, GRO‑ alpha, and MCP-1 (16, 22-25) while augmenting production of hematopoietic growth factors, such as G-CSF and GM-CSF (16, 26, 27), which promote the growth and maturation of the recruited myeloid cells. In addition, IL-17 serves as a bridge between innate and adaptive immune responses by enhancing the induction of co-stimulatory molecules such as ICAM-1 and other cytokines (16, 22, 28), thereby supporting T cell activation. IL-17 expression has been associated with many inflammatory diseases, such as rheumatoid arthritis, multiple sclerosis, asthma, systemic lupus erythematosus and allograft rejection (15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265. Kolls, J.K. and A. Linden (2004) Immunity 21:467. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625. Steinman, L. (2007) Nat. Med. 13:139. Hunter, C.A. (2005) Nat. Rev. Immunol.5:521. Lockhart, E. et al. (2006) J. Immunol. 177:4662. Ferretti, S. et al. (2003) J. Immunol. 170:2106. Shin, H.C. et al. (1999) Cytokine 11:257. Molet, S. et al. (2001) J. Allergy Clin. Immunol. 108:430. Zhou, Q. et al. (2005) Infect. Immun. 73:935. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462. Chang, S.H. and C. Dong (2007) Cell Res. 17:435. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593. Yao, Z. et al. (1995) Immunity 3:811. Attur, M.G. et al. (1997) Arthritis Rheum.40:1050. Ruddy, M.J. et al. (2004) J. Biol. Chem. 279:2559. Albanesi, C. et al. (1999) J. Immunol. 162:494. Witowski, J. et al. (2000) J. Immunol. 165:5814. Miyamoto, M. et al. (2003) J. Immunol. 170:4665. Ye, P. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:335. Laan, M. et al. (2001) Br. J. Pharmacol. 133:200. Starnes, T. et al. (2002) J. Immunol. 169:642. Jones, C.E. et al. (2002) Am. J. Respir. Cell Mol. Biol. 26:748. Yao, Z. et al. (1995) J. Immunol. 155:5483.

参考图片

IL-6 Secretion Induced by IL‑17 and Neutralization by Canine IL‑17 Antibody.

Recombinant Canine IL‑17 (Catalog # 5848-CL) induces IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). IL-6 Secretion elicited by Recombinant Canine IL‑17 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL‑17 Monoclonal Antibody (Catalog # MAB5848). The ND₅₀ is typically 5-30 ng/mL.