Mouse IL-17 RA/IL-17 R MAb (Clone 657603) (25 UG)【科瑞奥博】

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Mouse IL-17 RA/IL-17 R MAb (Clone 657603) (25 UG)

货号： MAB4481-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB4481-SP
别名	CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 receptor A; IL17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A
全称	Interleukin 17 Receptor
反应种属	Mouse
应用	Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-17 RA, recombinant mouse (rm) IL-17B R, rmIL-17 RD, or rmIL-17 RC is observed. In Western blots, less than 10% cross‑reactivity with recombinant human (rh) IL-17 RA or rhIL-17B R is observed.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑17-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND₅₀) is typically 0.2-0.8 μg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17.
来源	Monoclonal Rat IgG_2A Clone # 657603
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	23765 (Human); 16172 (Mouse); 312679 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function Proc Natl Acad Sci USA, 2016;0(0):. Species: Mouse Sample Type: In Vivo Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RA/IL‑17 R Ser32-Trp322 Accession # Q60943
生物活性	Mouse
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.5 mg/mL.
背景	IL-17 receptor alpha subunit (IL-17 RA), also known as IL-17 R, is a widely expressed 120 kDa glycosylated type I transmembrane protein that plays a central role in inflammatory responses. IL‑17 RA associates with IL-17 RC to form a signaling receptor complex for IL-17 and IL-17F. IL-17 RA ligation promotes T cell activation and the production of IL-6, G‑CSF, SCF, and multiple proinflammatory chemokines. Within the ECD, mouse IL-17 RA shares 84% and 72% aa sequence identity with rat and human IL17 RA, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokines and Receptors in Tfh Cell Differentiation and Function Human CD Marker Products IL-17 Family Mini-review: Interleukin 17 (IL-17) Multiple Sclerosis and Myelin Related Molecules Th17 Cells Th2 Cells Th9 Cells

参考图片

Detection of IL‑17 RA/IL‑17 R in EL-4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB4481, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

IL-6 secretion Induced by IL‑17 and Neutralization by Mouse IL‑17 RA/IL‑17 R Antibody. Recombinant Mouse IL‑17 (Catalog # 421-ML) induces IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL‑17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB4481). The ND₅₀ is typically 0.2‑0.8 μg/mL.