Human CCR3 MAb (Clone 61828) (25 UG)【科瑞奥博】

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Human CCR3 MAb (Clone 61828) (25 UG)

货号： MAB155-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB155-SP
简介	Clone 61828 was used by HLDA to establish CD designation
别名	b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the BaF3 mouse proB cell line transfected with human CCR3. The Neutralization Dose (ND₅₀) is typically 0.05-0.25 µg/mL in the presence of 0.333 µg/mL Recombinant Human CCL11/Eotaxin.
来源	Monoclonal Rat IgG_2A Clone # 61828
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Flow Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells Authors: Guichun Huang Sci Rep, 2016;6(0):27152. Species: Human Sample Type: Whole Cells Application: Neut Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Nature, 2011;475(7355):226-30. Species: Mouse Sample Type: Whole Cells Application: Neut Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J Int. J. Cancer, 2010;127(2):332-44. Species: Human Sample Type: Whole Cells Application: Neut Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D J. Immunol., 2008;181(11):8053-67. Species: Human Sample Type: Whole Cells Application: Flow The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J Stem Cells, 2007;25(7):1737-45. Species: Human Sample Type: Whole Cells Application: Flow Membrane-bound eotaxin-3 mediates eosinophil transepithelial migration in IL-4-stimulated epithelial cells. Authors: Yuan Q, Campanella GS, Colvin RA, Hamilos DL, Jones KJ, Mathew A, Means TK, Luster AD Eur. J. Immunol., 2006;36(10):2700-14. Species: Human Sample Type: Whole Cells Application: Neut Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Clin. Immunol., 2006;120(2):220-31. Species: Human Sample Type: Whole Cells Application: Neut Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion. Authors: Spencer LA, Melo RC, Perez SA, Bafford SP, Dvorak AM, Weller PF Proc. Natl. Acad. Sci. U.S.A., 2006;103(9):3333-8. Species: Human Sample Type: Whole Cells Application: Flow Functional analysis of the chemokine receptor CCR3 on airway epithelial cells. Authors: Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C J. Immunol., 2006;177(5):3344-54. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts. Authors: Puxeddu I, Bader R, Piliponsky AM, Reich R, Levi-Schaffer F, Berkman N J. Allergy Clin. Immunol., 2005;117(1):103-10. Species: Human Sample Type: Whole Tissue Application: IHC Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D J. Immunol., 2004;173(11):6592-602. Species: Human Sample Type: Whole Cells Application: Flow Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A J. Leukoc. Biol., 2003;73(5):584-90. Species: Human Sample Type: Whole Cells Application: Flow Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, RÃƒÂ©vÃƒÂ©sz T Blood, 2003;103(7):2806-8. Species: Human Sample Type: Whole Cells Application: Flow Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells. Authors: Howard OM, Dong HF, Yang D, Raben N, Nagaraju K, Rosen A, Casciola-Rosen L, Hartlein M, Kron M, Yiadom K, Dwivedi S, Plotz PH, Oppenheim JJ J. Exp. Med., 2002;196(6):781-91. Species: Human Sample Type: Whole Tissue Application: IHC Fresh/Frozen Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo. Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M Eur. J. Immunol., 2001;31(4):1037-46. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Y3 rat myeloid cell line transfected with human CCR3 Accession # P51677
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Basophils CC Chemokines and Receptors Chemokine Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Eosinophils Human CD Marker Products Myeloid Lineage Markers T Cell Migration/Adhesion Th2 Cells

参考图片

Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCR3 Antibody.

Recombinant Human CCL11/Eotaxin (Catalog # 320‑EO) chemoattracts the BaF3 mouse proB cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant CCL11/Eotaxin (0.333 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155). The ND₅₀ is typically 0.05-0.25 µg/mL.

CCR3 in Human Lymph Node.

CCR3 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS018) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.