| 货号 | MAB5357-SP |
| 别名 | carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; CHST4; EC 2.8.2; EC 2.8.2.-; EC 2.8.2.17; EC 2.8.2.21; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST2; GlcNAc6ST-2; gn6st-2; GST3; GST-3; HECGLCNAC6ST; HEC-GlcNAc6ST; HEC-GLCNAC-6-ST; High endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; LSSTcarbohydrate sulfotransferase 4; N-acetylglucosamine 6-O-sulfotransferase 2 |
| 反应种属 | Human |
| 应用 | Immunoprecipitation,Neutralization |
| 目标/特异性 | Detects human Carbohydrate Sulfotransferase 4/CHST4 is direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CHST1, 2, 5, 6, 10, 14, recombinant mouse CHST3, 4, and 7 is observed. |
| 使用方法 | Immunoprecipitation Neutralization |
| 来源 | Monoclonal Mouse IgG1 Clone # 595223 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10164 (Human); 26887 (Mouse); 307838 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Sulfotransferase 4/CHST4 His29-His386 Accession # Q8NCG5 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids. This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses. CHST4, also known as high endothelial cells N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L-selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L-selectin ligands. It has a catalytic preference for core 2-branched mucin-type O-glycans, but also has activity toward core 3 type of O-glycan. Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|