| 货号 | MAB8210-SP |
| 别名 | gastric inhibitory polypeptide receptor; GIP-R; Glucose-dependent insulinotropic polypeptide receptor; MGC126722; PGQTL2 | 全称 | Gastric Inhibitory Polypeptide Receptor |
| 反应种属 | Human |
| 应用 | Flow Cytometry(0.25 µg/106cells) Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human GIPR in direct ELISAs. Stains human GIPR transfected cells but not irrelevant transfectants. |
| 使用方法 | Flow Cytometry: 0.25 µg/106cells Immunohistochemistry: 8-25 µg/mL Blockade of Receptor-ligand Interaction: At 2.5 ug/mL, this antibody will block >95% of the binding. |
| 来源 | Monoclonal Mouse IgG1 Clone # 591853 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2696 (Human); 381853 (Mouse); 25024 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human GIPR. Met1-Cys466 Accession # P48546 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b‑cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins. | |
GIPR in Human Pancreas. GIPR was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
HumanGIP binding to human GIPR-transfected HEK293 cells blocked by Human GIPRAntibody. In a functional flow cytometry test, biotinylatedrecombinant Human GIP (Catalog # 2257,25 ng/mL) binds to Human GIPR-transfected HEK293 cells (black dotted line).Binding is completely blocked (orange histogram) by 2.5 μg/mL of MouseAnti-Human GIPR Monoclonal Antibody (Catalog # MAB8210). Mouse IgG1 IsotypeControl (Catalog # MAB002)at 2.5 μg/mL was used as a control (blue line). Cells were stained withSterptavidin-APC (Catalog # F0050). |
