| 货号 | MAB5265-SP |
| 别名 | C16orf77 | 全称 | Interleukin 34 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects recombinant human IL-34 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-34 is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 2.5 µg/106cells Neutralization: Measured by its ability to neutralize IL‑34-induced proliferation in human peripheral blood monocytes. Lin, H. et al. (2008) Science 320:807. The Neutralization Dose (ND50) is typically 0.01-0.05 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑34. |
| 来源 | Monoclonal Mouse IgG1 Clone # 578416 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 146433 (Human); 76527 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human IL-34 Asn21-Pro242 Accession # Q6ZMJ4 |
| 内毒素水平 | <1.0 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑34 and Neutralization by Human IL‑34 Antibody. Recombinant Human IL‑34 (Catalog # 5265-IL) stimulates proliferation in human peripheral blood monocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑34 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑34 Monoclonal Antibody (Catalog # MAB5265). The ND50 is typically 0.01-0.05 µg/mL. |
