| 货号 | MAB5348-SP |
| 别名 | Acid sphingomyelinase; aSMase; ASMsphingomyelin phosphodiesterase; EC 3.1.4.12; NPD; sphingomyelin phosphodiesterase 1, acid lysosomal | 全称 | Sphingomyelin Phosphodiesterase 1, Acid Lysosomal |
| 反应种属 | Human |
| 应用 | Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human SMPD1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SMPD3 and recombinant mouse SMPD1 is observed. |
| 使用方法 | Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human SMPD1 (0.5 µg/mL, Catalog # 5348-PD) cleavage of the substrate 2-N-Hexadecanoylamino- 4-nitrophenylphosphorylcholine (HNPPC, 250 µM). The Neutralization Dose (ND50) is typically 3.0 µg/mL |
| 来源 | Monoclonal Mouse IgG2A Clone # 563418 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6609 (Human); 20597 (Mouse); 308909 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human SMPD1 isoform 1 His62-Pro628 Accession # NP_000534 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Sphingomyelin phosphodiesterase, also known as acid sphingomyelinase and encoded by the SMPD1 gene, is a lysosomal phosphodiesterase which belongs to the acid sphingomyelinase family (1). SMPD1 catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. Ceramide, a bioactive lipid, has emerged as an important signaling molecule involved in a variety of cellular processes such as cell differentiation, apoptosis, and proliferation (2). Activation of SMPD1 occurs by the removal, chemical modification or dimerization of its C-terminal cysteine residue (3). Deficiencies of SMPD1 result in a lysosomal storage disorder referred to as Niemann-Pick disease (4). rhSMPD1 was expressed without the last three C-terminal residues, and is therefore constitutively active. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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