| 货号 | MAB20893-SP |
| 别名 | cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE-1XLKD1; XLKD1 | 全称 | Lymphatic Vessel Endothelial Hyaluronan Receptor 1 |
| 反应种属 | Human |
| 应用 | Blockade of Receptor-ligand Interaction |
| 目标/特异性 | Detects human LYVE-1 in direct ELISAs. |
| 使用方法 | Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.04-0.2 µg/mL of this antibody will block 50% of the binding of 1.5 μg/mL of biotinylated Hyaluronan to immobilized Recombinant Human LYVE-1 (Catalog # 2089-LY) coated at 5 µg/mL (100 µL/well). At 2 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Mouse IgG1 Clone # 537022 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10894 (Human); 114332 (Mouse); 293186 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human LYVE-1 Ser24-Thr238 Accession # Q9Y5Y7 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a60 - 70 kDa type I transmembrane protein that is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D‑glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1‑3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity. LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9‑11). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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