Mouse CCR7 MAb (Clone 4B12) (25 UG)【科瑞奥博】

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Mouse CCR7 MAb (Clone 4B12) (25 UG)

货号： MAB3477-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB3477-SP
别名	BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7chemokine (C-C) receptor 7; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor
反应种属	Mouse
应用	Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects mouse CCR7.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR7. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 25 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.
来源	Monoclonal Rat IgG_2A Clone # 4B12
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1236 (Human); 12775 (Mouse); 287673 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis. Authors: Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J Oncogene, 2015;35(6):748-60. FOXO1 regulates dendritic cell activity through ICAM-1 and CCR7. Authors: Dong G, Wang Y, Xiao W, Pacios Pujado S, Xu F, Tian C, Xiao E, Choi Y, Graves D J Immunol, 2015;194(8):3745-55. Species: Mouse Sample Type: Whole Cells Application: IF CC-Chemokine receptor CCR7: a key molecule for lymph node metastasis in esophageal squamous cell carcinoma. Authors: Irino, Tomoyuki, Takeuchi, Hiroya, Matsuda, Sachiko, Saikawa, Yoshiro, Kawakubo, Hirofumi, Wada, Norihito, Takahashi, Tsunehir, Nakamura, Rieko, Fukuda, Kazumasa, Omori, Tai, Kitagawa, Yuko BMC Cancer, 2014;14(0):291. Species: Human Sample Type: Whole Cell Application: ELISA Development Direct B cell stimulation by dendritic cells in a mouse model of lupus. Authors: Wan S, Zhou Z, Duan B, Morel L Arthritis Rheum., 2008;8(0):1741-50. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	RBC-2H3 cells expressing mouse CCR7 Accession # NP_031745
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCR7 is a 7TM G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers CC Chemokines and Receptors Chemokine Receptors Chemokines and Receptors in Angiogenesis Chemokines and Receptors in Tfh Cell Differentiation and Function Dendritic Cell Adhesion and Migration Human CD Marker Products ICC / IHC Images: CCR7 Regulatory T Cells (Tregs) T Cell Migration/Adhesion Technical Notes: CCR7

参考图片

CCR7 in Mouse Splenocytes. CCR7 was detected in immersion fixed non-stimulated mouse splenocytes using 25 µg/mL Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCR7 Antibody. Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP‑3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477). The ND₅₀ is typically 2-10 µg/mL.

Detection of CCR7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) or (B) Rat IgG_2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.