Human CXCR3 MAb (Clone 49801) (25 UG)【科瑞奥博】

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Human CXCR3 MAb (Clone 49801) (25 UG)

货号： MAB160-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB160-SP
别名	CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXCR3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human CXCR3.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CXCL11/I‑TAC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 7 ng/mL Recombinant Human CXCL11/I‑TAC.
来源	Monoclonal Mouse IgG₁ Clone # 49801
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2833 (Human); 12766 (Mouse); 84475 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TNF-? augments CXCR2 and CXCR3 to promote progression of renal cell carcinoma Authors: Kuang-Hui Sun J Cell Mol Med, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB CXCR3 Polymorphism and Expression Associate with Spontaneous Preterm Birth. Authors: Karjalainen M, Ojaniemi M, Haapalainen A, Mahlman M, Salminen A, Huusko J, Maatta T, Kaukola T, Anttonen J, Ulvila J, Haataja R, Teramo K, Kingsmore S, Palotie A, Muglia L, Ramet M, Hallman M J Immunol, 2015;195(5):2187-98. Species: Human Sample Type: Tissue Homogenates Application: WB Skin Metabolites Define a New Paradigm in the Localization of Skin Tropic Memory T Cells. Authors: McCully M, Collins P, Hughes T, Thomas C, Billen J, ODonnell V, Moser B J Immunol, 2015;195(1):96-104. Species: Human Sample Type: Whole Cells Application: Flow Role of CXCR3/CXCL10 axis in immune cell recruitment into the small intestine in celiac disease. Authors: Bondar C, Araya R, Guzman L, Rua E, Chopita N, Chirdo F PLoS ONE, 2014;9(2):e89068. Species: Human Sample Type: Whole Tissue Application: IF Dysregulation of CXCR3 expression on peripheral blood leukocytes in patients with neovascular age-related macular degeneration. Authors: Falk M, Singh A, Faber C, Nissen M, Hviid T, Sorensen T Invest Ophthalmol Vis Sci, 2014;55(7):4050-6. Species: Human Sample Type: Whole Cells Application: Flow Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis. Authors: Chaturvedi, Pallavi, Gilkes, Daniele, Wong, Carmen C, Luo, Weibo, Zhang, Huafeng, Wei, Hong, Takano, Naoharu, Schito, Luana, Levchenko, Andre, Semenza, Gregg L J Clin Invest, 2013;123(1):189-205. Species: Human Sample Type: Cell Lysates Application: WB Long-term Vaccination with Multiple Peptides Derived from Cancer-Testis Antigens Can Maintain a Specific T-cell Response and Achieve Disease Stability in Advanced Biliary Tract Cancer. Authors: Aruga A, Takeshita N, Kotera Y, Okuyama R, Matsushita N, Ohta T, Takeda K, Yamamoto M Clin Cancer Res, 2013;19(8):2224-31. Species: Human Sample Type: Whole Cells Application: Flow Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion. Authors: Wu Q, Dhir R, Wells A Mol. Cancer, 2012;11(0):3. Species: Human Sample Type: Cell Lysates Application: WB Ubiquitination of CXCR7 controls receptor trafficking. Authors: Canals M, Scholten DJ, de Munnik S, Han MK, Smit MJ, Leurs R PLoS ONE, 2012;7(3):e34192. Species: Human Sample Type: Whole Cells Application: Internalization Assay Identification of human NK17/NK1 cells. Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA PLoS ONE, 2011;6(10):e26780. Species: Human Sample Type: Whole Cells Application: Flow CXCR3 activation by lentivirus infection suppresses neuronal autophagy: neuroprotective effects of antiretroviral therapy. Authors: Zhu Y, Vergote D, Pardo C, Noorbakhsh F, McArthur JC, Hollenberg MD, Overall CM, Power C FASEB J., 2009;23(9):2928-41. Species: Feline Sample Type: Tissue Homogenates Application: Functional Assay Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease. Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS J. Immunol., 2009;182(6):3919-27. Species: Human Sample Type: Whole Tissue Application: IHC Frozen CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis. Authors: Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP, Strieter RM, Elashoff RM, Belperio JA Eur. Respir. J., 2009;34(3):676-86. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Authors: Franciszkiewicz K, Le Floch A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Cancer Res., 2009;69(15):6249-55. Species: Human Sample Type: Whole Cells Application: Flow Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D J. Immunol., 2008;181(11):8053-67. Species: Human Sample Type: Whole Cells Application: Flow The chemokine receptor CXCR3 is degraded following internalization and is replenished at the cell surface by de novo synthesis of receptor. Authors: Meiser A, Mueller A, Wise EL, McDonagh EM, Petit SJ, Saran N, Clark PC, Williams TJ, Pease JE J. Immunol., 2008;180(10):6713-24. Species: Human Sample Type: Whole Cells Application: Flow Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH J. Immunol., 2007;179(6):3724-33. Species: Human Sample Type: Whole Cells Application: Flow Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D J. Immunol., 2007;179(6):4272-82. Species: Human Sample Type: Whole Cells Application: ICC Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Clin. Immunol., 2006;120(2):220-31. Species: Human Sample Type: Whole Cells Application: Neut Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus. Authors: Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B Arthritis Rheum., 2005;52(5):1504-16. Species: Human Sample Type: Whole Tissue Application: IHC Fresh/Frozen Chemokine receptor expression in rat adjuvant-induced arthritis. Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE Arthritis Rheum., 2005;52(12):3718-30. Species: Rat Sample Type: Whole Tissue Application: IHC Up-regulation of the interferon gamma (IFN-gamma)-inducible chemokines IFN-inducible T-cell alpha chemoattractant and monokine induced by IFN-gamma and of their receptor CXC receptor 3 in human renal cell carcinoma. Authors: Suyama T, Furuya M, Nishiyama M, Kasuya Y, Kimura S, Ichikawa T, Ueda T, Nikaido T, Ito H, Ishikura H Cancer, 2005;103(2):258-67. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma. Authors: Srivastava BI, Srivastava MD Leuk. Res., 2005;29(7):771-83. Species: Human Sample Type: Whole Cells Application: ICC Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11. Authors: Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsic B J. Neuroimmunol., 2005;163(1):128-34. Species: Human Sample Type: Whole Cells Application: Flow Human immunodeficiency virus type 1 Nef protein mediates neural cell death: a neurotoxic role for IP-10. Authors: van Marle G, Henry S, Todoruk T, Sullivan A, Silva C, Rourke SB, Holden J, McArthur JC, Gill MJ, Power C Virology, 2004;329(2):302-18. Species: Human Sample Type: Whole Cells Application: ICC Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. Authors: Lim HW, Hillsamer P, Kim CH J. Clin. Invest., 2004;114(11):1640-9. Species: Human Sample Type: Whole Cells Application: ICC Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis. Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M Int. J. Oncol., 2004;25(3):605-13. Species: Human Sample Type: Whole Cells Application: ICC Expression of functional CCR and CXCR chemokine receptors in podocytes. Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H J. Immunol., 2002;168(12):6244-52. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo. Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M Eur. J. Immunol., 2001;31(4):1037-46. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	NS0 mouse myeloma cell line transfected with human CXCR3 Met1-Leu368 Accession # P49682
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCR3 is a G protein-coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10), and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Chemotaxis, Migration, and Adhesion Chemokine Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Group 1 ILCs Human CD Marker Products Myeloid Lineage Markers Neuroinflammation Regulatory T Cells (Tregs) T Cell Migration/Adhesion Th1 Cells

参考图片

Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCR3 Antibody. Recombinant Human CXCL11/I‑TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (7 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160). The ND₅₀ is typically 0.3-1.5 µg/mL.

CXCR3 in Human Tonsil. CXCR3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Human CXCR3 Monoclonal Antibody (Catalog # MAB160) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.