Human CXCR2/IL-8 RB MAb (Clone 48311) (25 UG)【科瑞奥博】

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Human CXCR2/IL-8 RB MAb (Clone 48311) (25 UG)

货号： MAB331-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB331-SP
简介	Clone 48311 was used by HLDA to establish CD designation
别名	beta; CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B
全称	Interleukin 8 Receptor B
反应种属	Human
应用	Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 5 ng/mL Recombinant Human CXCL1/GRO alpha /KC/CINC‑1.
来源	Monoclonal Mouse IgG_2A Clone # 48311
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3579 (Human); 12765 (Mouse); 29385 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TNF-? augments CXCR2 and CXCR3 to promote progression of renal cell carcinoma Authors: Kuang-Hui Sun J Cell Mol Med, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Flow CXCR2-CXCL1 axis is correlated with neutrophil infiltration and predicts a poor prognosis in hepatocellular carcinoma. Authors: Li L, Xu L, Yan J, Zhen Z, Ji Y, Liu C, Lau W, Zheng L, Xu J J Exp Clin Cancer Res, 2015;34(0):129. Species: Human Sample Type: Whole Tissue Application: IHC - Paraffin embedded CXCL1/CXCL8 (GROalpha/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro. Authors: Omatsu T, Cepinskas G, Clarson C, Patterson E, Alharfi I, Summers K, Couraud P, Romero I, Weksler B, Fraser D Am J Physiol Endocrinol Metab, 2014;306(9):E1077-84. Species: Human Sample Type: Whole Cells Application: Neut Tumor-derived interleukin-1 promotes lymphangiogenesis and lymph node metastasis through M2-type macrophages. Authors: Watari K, Shibata T, Kawahara A, Sata K, Nabeshima H, Shinoda A, Abe H, Azuma K, Murakami Y, Izumi H, Takahashi T, Kage M, Kuwano M, Ono M PLoS ONE, 2014;9(6):e99568. Species: Human Sample Type: Whole Cells Application: Neut Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche. Authors: Infanger, David W, Cho, YouJin, Lopez, Brina S, Mohanan, Sunish, Liu, S Chris, Gursel, Demirkan, Boockvar, John A, Fischbach, Claudia Cancer Res, 2013;73(23):7079-89. Species: Human Sample Type: Cell Lysates Application: WB Interleukin-33 overexpression is associated with gamma-glutamyl transferase in biliary atresia. Cytokine, 2011;61(2):433-7. Species: Human Sample Type: Whole Cells Application: Neut Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer. Authors: Li A, King J, Moro A, Sugi MD, Dawson DW, Kaplan J, Li G, Lu X, Strieter RM, Burdick M, Go VL, Reber HA, Eibl G, Hines OJ Am. J. Pathol., 2011;178(3):1340-9. Species: Human Sample Type: Whole Cells Application: Neut Overexpression of the beta subunit of human chorionic gonadotropin promotes the transformation of human ovarian epithelial cells and ovarian tumorigenesis. Authors: Guo X, Liu G, Schauer IG, Yang G, Mercado-Uribe I, Yang F, Zhang S, He Y, Liu J Am. J. Pathol., 2011;179(3):1385-93. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Reduced expression of chemoattractant receptors by polymorphonuclear leukocytes in Hyper IgE Syndrome patients. Authors: Mintz R, Garty BZ, Meshel T, Marcus N, Katanov C, Cohen-Hillel E, Ben-Baruch A Immunol. Lett., 2010;130(1):97-106. Species: Human Sample Type: Whole Cells Application: Flow CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis. Authors: Yang G, Rosen DG, Liu G Clin. Cancer Res., 2010;16(15):3875-86. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O Eur. J. Haematol., 2010;84(3):239-51. Species: Human Sample Type: Whole Cells Application: Neut Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis. Authors: van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P Clin. Exp. Immunol., 2009;158(1):143-53. Species: Human Sample Type: Whole Cells Application: Neut CXCR1 and CXCR2 enhances human melanoma tumourigenesis, growth and invasion. Authors: Singh S, Nannuru KC, Sadanandam A, Varney ML, Singh RK Br. J. Cancer, 2009;100(10):1638-46. Species: Human Sample Type: Cell Lysates Application: WB Potential role of high-mobility group box 1 in cystic fibrosis airway disease. Authors: Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, ONeal W, Sorscher EJ, Abraham E, Blalock JE Am. J. Respir. Crit. Care Med., 2008;178(8):822-31. Species: Human Sample Type: Whole Cells Application: Neut TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche. Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H Cancer Res., 2008;68(23):9754-62. Species: Mouse Sample Type: In Vivo Application: In vivo Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events. Authors: Gouwy M, Struyf S, Noppen S, Schutyser E, Springael JY, Parmentier M, Proost P, Van Damme J Mol. Pharmacol., 2008;74(2):485-95. Species: Human Sample Type: Whole Cells Application: Flow Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina. Authors: Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M Invest. Ophthalmol. Vis. Sci., 2008;49(10):4578-89. Species: Human Sample Type: Whole Tissue Application: IHC Fresh A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation. Authors: Gaggar A, Jackson PL, Noerager BD, OReilly PJ, McQuaid DB, Rowe SM, Clancy JP, Blalock JE J. Immunol., 2008;180(8):5662-9. Species: Mouse Sample Type: Whole Cells Application: Neut Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells. Authors: Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR Crit. Care Med., 2007;35(3):879-85. Species: Human Sample Type: Whole Cells Application: Neut Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression. Authors: Kline M, Donovan K, Wellik L, Lust C, Jin W, Moon-Tasson L, Xiong Y, Witzig TE, Kumar S, Rajkumar SV, Lust JA Leuk. Res., 2007;31(5):591-8. Species: Human Sample Type: Whole Cells Application: Flow Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. Authors: Smith C, Damas JK, Otterdal K, ÃƒËœIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P J. Am. Coll. Cardiol., 2006;48(8):1591-9. Species: Human Sample Type: Whole Cells Application: Neut The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium. Authors: Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M J. Neuroimmunol., 2005;161(1):49-60. Species: Guinea Pig Sample Type: Whole Cells Application: ICC Chemokine receptor expression in rat adjuvant-induced arthritis. Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE Arthritis Rheum., 2005;52(12):3718-30. Species: Rat Sample Type: Whole Tissue Application: IHC IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H J. Immunol., 2005;175(9):6177-89. Species: Human Sample Type: Cell Culture Supernates Application: Neut Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2. Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE J. Leukoc. Biol., 2004;76(2):441-50. Species: Human Sample Type: Whole Cells Application: Neut Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, RÃƒÂ©vÃƒÂ©sz T Blood, 2003;103(7):2806-8. Species: Human Sample Type: Whole Cells Application: Flow Expression of functional CCR and CXCR chemokine receptors in podocytes. Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H J. Immunol., 2002;168(12):6244-52. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome. Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM J. Immunol., 2002;169(11):6515-21. Species: Human Sample Type: BALF Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB Met1-Leu355 Accession # AAB25880
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chemokine Receptors Chemokines and Receptors in Angiogenesis Human CD Marker Products Macrophage Activation Markers Megakaryocytes Neuroinflammation Neutrophils T Cell Migration/Adhesion

参考图片

Detection of CXCR2/IL‑8 RB in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human
CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

CXCR2/IL‑8 RB in Human Lymph Node. CXCR2/IL‑8 RB was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Mouse Anti-Human CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCR2/IL‑8 RB Antibody. Recombinant Human CXCL1/GRO alpha (Catalog #
275-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL1/GRO alpha (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human
CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331). The ND₅₀ is typically 1-5 µg/mL.