Human CCR5 MAb (Clone 45531) (25 UG)【科瑞奥博】

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Human CCR5 MAb (Clone 45531) (25 UG)

货号： MAB182-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB182-SP
别名	C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human CCR5.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 5-20 µg/mL in the presence of 40 ng/mL Recombinant Human CCL3/MIP‑1 alpha isoform LD78a.
来源	Monoclonal Mouse IgG_2B Clone # 45531
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer. Authors: Ward, S T, Li, K K, Hepburn, E, Weston, C J, Curbishley, S M, Reynolds, G M, Hejmadi, R K, Bicknell, R, Eksteen, B, Ismail, T, Rot, A, Adams, D H Br J Cancer, 2015;112(2):319-28. Species: Human Sample Type: Whole Tissue Application: IHC - paraffin CCR5 is a receptor for Staphylococcus aureus leukotoxin ED. Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J Nature, 2013;493(7430):51-5. Species: Human Sample Type: whole cells Application: Bioassay Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J Int. J. Cancer, 2010;127(2):332-44. Species: Human Sample Type: Whole Cells Application: Neut Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Authors: Franciszkiewicz K, Le Floch A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Cancer Res., 2009;69(15):6249-55. Species: Human Sample Type: Whole Cells Application: Flow Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions. Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A Int. J. Cancer, 2008;122(4):769-76. Species: Human Sample Type: Whole Cells Application: Flow Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D J. Immunol., 2008;181(11):8053-67. Species: Human Sample Type: Whole Cells Application: Flow Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction. Authors: Bolovan-Fritts CA, Spector SA Blood, 2007;111(1):175-82. Species: Human Sample Type: Whole Cells Application: Neut Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH J. Immunol., 2007;179(6):3724-33. Species: Human Sample Type: Whole Cells Application: Flow Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Clin. Immunol., 2006;120(2):220-31. Species: Human Sample Type: Whole Cells Application: Neut Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells. Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS Cytokine, 2006;36(5):237-44. Species: Human Sample Type: Whole Cells Application: Neut Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11. Authors: Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsic B J. Neuroimmunol., 2005;163(1):128-34. Species: Human Sample Type: Whole Cells Application: Flow CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2. Authors: Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F Cell. Signal., 2005;17(3):355-63. Species: Human Sample Type: Whole Cells Application: Flow Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. Authors: Lim HW, Hillsamer P, Kim CH J. Clin. Invest., 2004;114(11):1640-9. Species: Human Sample Type: Whole Cells Application: ICC Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A J. Leukoc. Biol., 2003;73(5):584-90. Species: Human Sample Type: Whole Cells Application: Neut Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells. Authors: Howard OM, Dong HF, Yang D, Raben N, Nagaraju K, Rosen A, Casciola-Rosen L, Hartlein M, Kron M, Yiadom K, Dwivedi S, Plotz PH, Oppenheim JJ J. Exp. Med., 2002;196(6):781-91. Species: Human Sample Type: Whole Cells Application: Neut Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function. Authors: Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW J. Biol. Chem., 1999;274(14):9617-26. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	NS0 mouse myeloma cell line transfected with human CCR5 Met1-Leu352 Accession # P51681
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for macrophage tropic HIV-1 infection.
运输条件	Blue Ice
存放说明	4℃
参考文献	CC Chemokines and Receptors Chemokine Receptors Chemokines and Receptors in Angiogenesis Common Macrophage Markers Cytokine Upregulation of HIV Coreceptors gamma delta T Cells Human CD Marker Products Infectious Diseases Monocyte Markers Multiple Myeloma Neuroinflammation Receptors in the Jak/STAT Pathway Regulatory T Cells (Tregs) T Cell Migration/Adhesion T. gondii: A Therapeutic Agent for HIV-1? Th1 Cells

参考图片

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Human CCR5 Antibody. Recombinant Human CCL3/MIP‑1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP‑1 alpha (40 ng/mL) is neutralized (green line) by increasing concentrations of Human CCR5 Monoclonal Antibody (Catalog # MAB182). The ND₅₀ is typically 5-20 µg/mL.

CCR5 in Human Dendritic Cells. CCR5 was detected in immersion fixed human dendritic cells using Human CCR5 Monoclonal Antibody (Catalog # MAB182) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.