| 货号 | MAB4009-SP |
| 别名 | ALPBRE; BCAN; BEHAB; BEHABbrevican core protein; Brain-enriched hyaluronan-binding protein; brevican; Chondroitin sulfate proteoglycan 7; chondroitin sulfate proteoglycan BEHAB; chondroitin sulfate proteoglycan BEHAB/brevican; CSPG7; CSPG7brevican proteoglycan; MGC13038 | 全称 | Brain Specific Proteoglycan in the Aggrecan Family |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Brevican in direct ELISAs and Western blots. Also detects a truncated version of recombinant human Brevican (aa 23‑649). |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.01-0.05 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated Hyaluronan (132 kDa) to immobilized Recombinant Human Brevican (Catalog # 4009-BC) coated at 3 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Mouse IgG1 Clone # 450613 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 63827 (Human); 12032 (Mouse); 25393 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Brevican isoform 1 Asp23-Pro911 Accession # AAH09117 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Brevican, also called BEHAB, is a secreted member of the the lectican family of proteoglycans that share a common domain structure (1). Brevican contains an Ig‑like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain (2). Brevican is restricted to the CNS and is expressed by astrocytes, oligodendrocytes, and neurons (3‑7). A GPI-anchored alternate splice form exists that is truncated following the central (GAG) region (2, 8). An additional isoform results from an alternate start site at Met228. Brevican is cleaved by multiple proteases and exists in a number of distinct fragments (5, 9, 10). Full-length brevican consists of a 97 kDa core protein with up to approximately 100 kDa of attached chondroitin sulfate but not heparan sulfate chains (4, 7, 11, 12). Brevican associates with the extracellular matrix, perineuronal nets, and astrocyte cell surfaces by means of its chondroitin sulfate, GPI anchor, hyaluronic acid-binding link domains, and the core protein (4, 7, 8, 13). The secreted isoform is dominant during brain development and is upregulated in astrocytes following brain injury (2, 14). In human and rat, an under-glycosylated form of brevican is upregulated in highly aggressive glioma but not in low-grade glioma or other brain pathologies (15, 16). In mouse and rat, levels of an ADAMTS4-generated 55 kDa N‑terminal fragment increase during remodeling after excitotoxic injury (11, 12). Human brevican shares 90%, 80%, and 80% aa sequence identity with bovine, mouse, and rat brevican, respectively. Within the Ig‑like and two link domains, brevican shares 45%‑51% aa sequence identity with aggrecan, neurocan, and versican. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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