| 货号 | MAB3710-SP |
| 别名 | Brain trypsinogen; EC 3.4.21; EC 3.4.21.4; Mesotrypsin; mesotrypsinogen; MTG; pancreatic trypsinogen III; protease, serine, 3 (mesotrypsin); protease, serine, 3; protease, serine, 4 (trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3MTG; TRY4mesotrypsin; trypsin 3; Trypsin 4; Trypsin III; Trypsin IV; trypsin-3; Trypsinogen 15; trypsinogen 4; trypsinogen 5; trypsinogen IV |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human Trypsin 3/PRSS3 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Trypsin-1 or rhTrypsin-2 is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize Recombinant Human Active Trypsin 3/PRSS3 (0.03 µg/mL, Catalog # 3714-SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 0.15 µg/mL. |
| 来源 | Monoclonal Rat IgG2A Clone # 420025 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5646 (Human); 22073 (Mouse); 362347 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Trypsin 3/PRSS3 Val16-Ser247 Accession # NP_002762 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin (1). Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas (2). Compared to trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198 (3). Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One proposed physiological function of Trypsin 3 is degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Neutralization of Trypsin 3/ PRSS3 Activity by Human Trypsin 3/PRSS3 Antibody. The cleavage of Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002) by Recombinant Human Active Trypsin 3/PRSS3 (0.03 µg/mL, Catalog # 3714-SE) is measured after preincubation with increasing concentrations of Rat Anti-Human Trypsin 3/PRSS3 Monoclonal Antibody (Catalog # MAB3710). The ND50 is typically 0.15 µg/mL. |
