Human/Primate IL-17 MAb (Clone 41809) (25 UG)【科瑞奥博】

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Human/Primate IL-17 MAb (Clone 41809) (25 UG)

货号： MAB317-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB317-SP
别名	CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
全称	Interleukin 17
反应种属	Human
应用	Immunoprecipitation,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human and primate IL-17 in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17 is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
使用方法	Immunoprecipitation: 3 µg/100 µg cell lysate ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.
来源	Monoclonal Mouse IgG_2B Clone # 41809
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage. Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S Eur J Immunol, 2015;46(2):440-5. Species: Human Sample Type: Whole Cells Application: Neut Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Authors: Todd J, Simpson P, Estis J, Torres V, Wub A Cytokine, 2013;64(3):660-5. Species: Human Sample Type: Plasma Application: ELISA Development TH17 cytokines induce human airway smooth muscle cell migration. Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2. Species: Human Sample Type: Whole Cells Application: Neut IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients. Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK Immunol. Lett., 2010;127(2):150-6. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes. Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P J. Immunol., 2009;182(0):3112-20. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Cytokine-induced CEACAM1 expression on keratinocytes is characteristic for psoriatic skin and contributes to a prolonged lifespan of neutrophils. Authors: Rahmoun M, Moles JP, Pedretti N, Mathieu M, Fremaux I, Raison-Peyron N, Lecron JC, Yssel H, Pene J J. Invest. Dermatol., 2009;129(3):671-81. Species: Human Sample Type: Whole Cells Application: Neut Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL J. Immunol., 2008;181(6):4299-310. Species: Human Sample Type: Whole Cells Application: Neut Ultrasensitive flow-based immunoassays using single-molecule counting. Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Clin. Chem., 2007;53(11):1990-5. Species: Human Sample Type: Plasma Application: ELISA Development Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. Authors: Pfleger C, Schloot N, ter Veld F J. Immunol. Methods, 2007;329(1):214-8. Species: Human Sample Type: Serum Application: Luminex Development IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H J. Immunol., 2005;175(9):6177-89. Species: Human Sample Type: Whole Cells Application: Neut IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate. Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E J. Immunol., 2004;173(2):1463-76. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human IL-17 Ile20-Ala155 Accession # Q16552
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Primate
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-17 is a pro-inflammatory cytokine secreted by activated T cells. Itis the prototype member of the IL-17 family that also includes IL-17B,C, D, E, and F.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Chemotaxis, Migration, and Adhesion Cytokines and Receptors in Tfh Cell Differentiation and Function Development and Characterization of Monoclonal Antibody Clone 41802 to Human IL-17A gamma delta T Cells Helper T (Th) Cells IL-17 Family IL-17 is Produced by T-cells Interactive Pathway: IL-17 Family Signaling Pathways LTi Cells Macrophage Activation Markers Mini-review: Interleukin 17 (IL-17) Multiple Sclerosis and Myelin Related Molecules Natural Killer T (NKT) Cells NCR-ILC3 Neuroinflammation T Cell Cytokine Signaling Technical Note: IL-17 Family Antibodies for Flow Cytometry Th17 Cells Th17 Differentiation: An Evolving Target for Multiple Sclerosis Therapy

参考图片

Immunoprecipitation of Human IL‑17.

Human IL-17 was immunoprecipitated from 100 μg of human primary differentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317) or isotype control antibody (Catalog # MAB004) overnight at 4 °C. IL-17-antibody complexes were absorbed using anti-mouse agarose beads. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

IL‑6 Secretion Induced by
IL‑17 and Neutralization by Human IL‑17 Antibody.

Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Human IL‑17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL‑17 Monoclonal Antibody (Catalog # MAB317). The ND₅₀ is typically 1-3 µg/mL.