Mouse IL-1 alpha/IL-1F1 MAb (Clone 40508) (25 UG)【科瑞奥博】

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Mouse IL-1 alpha/IL-1F1 MAb (Clone 40508) (25 UG)

货号： MAB400-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB400-SP
别名	Hematopoietin-1; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
全称	Interleukin 1 alpha
反应种属	Mouse
应用	ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse IL-1 alpha /IL-1F1 in ELISAs. In ELISAs, less than 1% cross-reactivity with recombinant rat IL‑1 alpha /IL‑1F1, recombinant porcine IL‑1 alpha /IL‑1F1, and recombinant human IL‑1 alpha /IL‑1F1 is observed.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.2-0.6 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.
来源	Monoclonal Rat IgG_2B Clone # 40508
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Alternatively activated macrophages determine repair of the infarcted adult murine heart J Clin Invest, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice. Authors: Dankbar B, Fennen M, Brunert D, Hayer S, Frank S, Wehmeyer C, Beckmann D, Paruzel P, Bertrand J, Redlich K, Koers-Wunrau C, Stratis A, Korb-Pap A, Pap T Nat Med, 2015;21(9):1085-90. Species: Mouse Sample Type: Whole Cells Application: Neut Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA J. Biol. Chem., 2009;0(0):. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Viral infection of the lungs through the eye. Authors: Bitko V, Musiyenko A, Barik S J. Virol., 2007;81(2):783-90. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse IL-1 alpha /IL-1F1 Ser115-Ser270 Accession # P01582
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta ‑converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines. IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4, and IL-13.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Markers Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis ICC / IHC Images: IL-1 alpha/IL-1F1 IL-1 Family Inflammasomes: Intracellular Regulators of Pathogen Interactive Pathway: IL-1 Family Signaling Pathways Megakaryocytes Mini-review: Interleukin 1 Neuroinflammation Regulatory T Cells (Tregs) T Cell Cytokine Signaling

参考图片

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Mouse
IL‑1 alpha /IL‑1F1 Antibody. Recombinant Mouse
IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB400). The ND₅₀ is typically 0.2-0.6 µg/mL in the presence of concanavalin A (1.25 µg/mL).