| 货号 | MAB3559-SP |
| 别名 | CHI3; CHIT1; CHITCHI3; chitinase 1 (chitotriosidase); chitinase-1; chitotriosidase-1; EC 3.2.1.14; FLJ00314; MGC125322; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase |
| 反应种属 | Human |
| 应用 | Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Chitotriosidase/CHIT1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CHI3L1 is observed. |
| 使用方法 | Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Chitotriosidase/CHIT1 (20 ng/mL, Catalog # 3559-GH) cleavage of the fluorogenic peptide substrate 4-Methylumbelliferyl-beta -D-N,N’,N”-triacetylchitotriose (4-MU-TACT) (0.01 mM). The Neutralization Dose (ND50) is typically 56 ng/mL. |
| 来源 | Monoclonal Mouse IgG1 Clone # 397504 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1118 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Chitotriosidase/CHIT1 Ala22-Asn466 Accession # Q13231 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Chitotriosidase, encoded by the CHIT1 gene, is a member of the chitinase family of endo-beta-1,4-linked N-acetylglucosaminases that cleave chitin, a structural component of cell walls and coatings of many organisms. Chitotriosidase is selectively expressed by activated tissue macrophages. Very high plasma levels of Chitotriosidase are found in patients with Gaucher disease type 1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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