Mouse CCL3/MIP-1 alpha MAb (Clone 39624) (25 UG)【科瑞奥博】

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Mouse CCL3/MIP-1 alpha MAb (Clone 39624) (25 UG)

货号： MAB450-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB450-SP
别名	C-C motif chemokine 3; MIP1-(a); AI323804; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3
反应种属	Mouse
应用	Western Blot(1 µg/mL)
目标/特异性	Detects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/MIP‑1 beta is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed. Neutralizes the biological activity of recombinant mouse CCL3 and will not block the biological activity of recombinant human CCL3.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically ≤10 µg/mL in the presence of 1 ng/mL Recombinant Mouse CCL3/MIP‑1 alpha.
来源	Monoclonal Rat IgG_2A Clone # 39624
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma. Authors: Deepak P, Kumar S, Kishore D, Acharya A Int. Immunol., 2010;22(1):53-63. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Authors: Fujita H, Asahina A, Komine M, Tamaki K Cell. Immunol., 2007;245(2):70-9. Species: Mouse Sample Type: Whole Cells Application: Neut Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions. Authors: Yang L, Mosmann T Eur. J. Immunol., 2004;34(6):1617-26. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions. Authors: Mosmann T Eur. J. Immunol., 2004;34(6):1617-26. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection. Authors: Reading PC, Symons JA, Smith GL J. Immunol., 2003;170(3):1435-42. Species: Mouse Sample Type: BALF Application: ELISA Development Regulatory effects of eotaxin on acute lung inflammatory injury. Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA J. Immunol., 2001;166(8):5208-18. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse CCL3/MIP-1 alpha Ala24-Ala92 Accession # P10855
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta (C‑C) subfamily of chemokines. Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8⁺ and CD4⁺ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.
运输条件	Blue Ice
存放说明	4℃
参考文献	Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

参考图片

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Mouse CCL3/
MIP‑1 alpha Antibody.

Recombinant Mouse CCL3/MIP‑1 alpha (Catalog # 450‑MA) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by Hema3 Staining System (Fisher Scientific). Chemotaxis elicited by Recombinant Mouse CCL3/MIP‑1 alpha (100 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB450). The ND₅₀ is typically 5-25 µg/mL.