| 货号 | MAB2896-SP |
| 别名 | carboxypeptidase A2 (pancreatic); carboxypeptidase A2; CPA2; EC 3.4.17; EC 3.4.17.15 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Carboxypeptidase A2/CPA2 in direct ELISAs and Western blots. In Western blots, recognizes the pro form but not the activated enzyme. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Carboxypeptidase A2/CPA2 (0.1 µg/mL, Catalog # 2896‑ZN) cleavage of the fluorogenic peptide substrate N-acetyl-Phe-Thiaphe-OH (0.1 mM). The Neutralization Dose (ND50) is typically 1.5 µg/mL. |
| 来源 | Monoclonal Mouse IgG2B Clone # 384014 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1358 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A2/CPA2 Tyr17-His417 Accession # P48052 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (residues 1 to 16), a pro region (residues 17 to 112), and a mature chain (residues 113 to 417). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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