Human TLR2 MAb (Clone 383936) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human TLR2 MAb (Clone 383936) (25 UG)

货号： MAB2616-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB2616-SP
别名	CD282 antigen; CD282; TIL4CD282; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2
全称	Toll-like Receptor 2
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Stains human TLR2 transfectants but not irrelevant transfectants.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize Pam₃CSK₄-induced IL‑8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2. The Neutralization Dose (ND₅₀) is typically 0.03-0.15 µg/mL in the presence of 0.5 µg/mL The synthetic tripalmitoylated lipopeptide Pam₃CSK₄.
来源	Monoclonal Mouse IgG_2B Clone # 383936
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7097 (Human); 24088 (Mouse); 310553 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells J Immunol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut Vaccine-Mediated Activation of Human TLR4 Is Affected by Modulation of Culture Conditions during Whole-Cell Pertussis Vaccine Preparation PLoS ONE, 2016;11(8):e0161428. Species: Human Sample Type: Whole Cells Application: Neut Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells. Authors: Brummelman J, Veerman R, Hamstra H, Deuss A, Schuijt T, Sloots A, Kuipers B, van Els C, van der Ley P, Mooi F, Han W, Pinelli E Infect Immun, 2015;83(1):227-38. Species: Human Sample Type: Whole Cells Application: Blocking Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses. Authors: Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G Innate Immun, 2012;18(3):531-40. Species: Human Sample Type: Cell Lysates Application: WB Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne. Authors: Lumsden KR, Nelson AM, Dispenza MC, Gilliland KL, Cong Z, Zaenglein AL, Thiboutot DM Br. J. Dermatol., 2011;165(2):302-10. Species: Human Sample Type: Whole Cells Application: Neut Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells. Authors: Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M Clin. Exp. Immunol., 2009;156(2):285-93. Species: Human Sample Type: Saliva Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	NS0 mouse myeloma cell line transfected with human TLR2 Accession # O60603
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Human toll-like receptor (TLR) family includes ten members that activate the innate immune response via an ability to recognize molecular structures found in a variety of microbial pathogens (1‑3). All TLR family members are type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid (aa) precursor (2) that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells endothelial cells, and hepatocytes (1, 2, 4). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors (1). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers bind triacylated lipopeptides, while the TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (5). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 6). TLR2 signaling results in dendritic cell maturation characterized by increased surface expression of class II MHC and the T cell costimulators, CD80 and CD86 (1, 2). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1-3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Wetzler, L. (2003) Vaccine 21:S2/55. Kirschning, C. and R. Schumann (2002) Curr. Top. microbiol. Immunol. 270:121. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749. Flo, T. et al. (2001) J. Leukoc. Biol. 69:474. Akira, S. (2003) Curr. Opin. Immunol. 15:5. Yamamoto, M. et al. (2002) Nature 420:324.

参考图片

IL‑8 Secretion Induced by Pam₃CSK₄ and Neutralization by Human TLR2 Antibody. The synthetic tripalmitoylated lipopeptide Pam₃CSK₄ stimulates IL‑8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL‑8 Quantikine ELISA Kit (Catalog # D8000C). IL‑8 secretion elicited by Pam₃CSK₄ (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616). The ND₅₀ is typically 0.03-0.15 µg/mL.

Detection of TLR2 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0102B).