| 货号 | MAB2529-SP |
| 别名 | CD97 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human CD97 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse CD97 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Neutralization: Measured by its ability to neutralize CD97-mediated adhesion of human red blood cells. Hamann, J. et al. (1996) J. Exp. Med. 184:1185. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 4 µg/mL Recombinant Human CD97. |
| 来源 | Monoclonal Mouse IgG2A Clone # 380903 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 976 (Human); 26364 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human CD97 Gln21-Gln398 Accession # NP_001775.2 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CD97 is a 95‑100 kDa member of a protein group known as the LNB-TM7 protein family that evolved from genes of the secretin receptor superfamily (1‑3). Molecules in this family are unique hybrid structures consisting of EGF-like modules coupled to class B G-protein 7-transmembrane (TM) domains by a glycosylated (mucin) stalk. Human CD97 is synthesized as an 835 amino acid (aa) precursor that contains a 20 aa signal sequence, a 532 aa extracellular domain (ECD), a 238 aa “transmembrane” region that includes seven TM segments, and a 45 aa cytoplasmic tail (4). Within the 532 aa ECD, the first 236 aa contains five EGF-like domains, the C-terminal four of which bind calcium, and a juxtamembrane 296 aa RGD-containing mucin stalk (4, 5). The stalk is both glycosylated and proteolytically cleaved (at aa 530) to create a noncovalently linked 65‑70 kDa glycosylated extracellular alpha -subunit and a 28 kDa 7-TM membrane-bound beta -subunit (4). There are two known alternate splice forms in human. Isoform # 1 contains four EGF-like domains (domain # 1, 2, 3 and 5), while isoform # 2 contains three EGF-like domains (domain # 1, 2 and 5) (1, 4, 6). The ECD in isoform 1 is 60 kDa in size, while the ECD in isoform 2 is 55 kDa in size (native molecular weight). The five EGF-like domain region in human is 55% aa identical to that in mouse. Cells known to express CD97 include monocytes, macrophages, T cells, select B cells, dendritic cells and, potentially, vascular and visceral smooth muscle cells (1, 7). There are at least two ligands for CD97. One is chrondroitin sulfate that binds only to the full-length (five domain) form of CD97. Binding is dependent on the presence of EGF-like domain # 4 (3). The second ligand for CD97 is CD55, a GPI-linked cell surface molecule with short consensus repeats that regulates complement activation on cell surfaces (1, 5, 7). CD97 EGF-like domains # 1 and 2 bind CD55 while domain # 5 stabilizes the CD97 molecule. The shortest CD97 isoform shows the strongest binding to CD55 (7, 8). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Adhesion Mediated by CD97 and Neutralization by Human CD97 Antibody. Recombinant Human CD97 (Catalog # 2529‑CD), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human CD97 (4 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD97 Monoclonal Antibody (Catalog # MAB2529). The ND50 is typically 0.5-2.5 µg/mL. | |
Detection of CD97 in Human Whole Blood by Flow Cytometry. Human whole blood was stained with Mouse Anti-Human CD97 Monoclonal Antibody (Catalog # MAB2529, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody (Catalog # F0102B). |
