| 货号 | MAB17191-SP |
| 别名 | EC 3.4.21; EC 3.4.21.118; hK8; HNP; kallikrein 8 (neuropsin/ovasin); kallikrein-related peptidase 8; KLK8 protein type 1; KLK8 protein type 2; KLK8; neuropsin type 1; neuropsin type 2; Neuropsin; NP; NRPN; Ovasin; PRSS19; PRSS19kallikrein-8; Serine protease 19; Serine protease TADG-14; TADG14neuropsin; Tumor-associated differentially expressed gene 14 protein |
| 反应种属 | Human |
| 应用 | Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Kallikrein 8/Neuropsin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) UPA‑1, rhKallikrein 1, 2, 3, 4, 5, 7, 9, 10, 11, 12, 13, 14, 15, rhKallikrein B1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI, rhProc, or rhThrombin is observed. |
| 使用方法 | Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Kallikrein 8/Neuropsin (0.5 µg/mL, Catalog # 2025-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-Amc (0.1 mM, Catalog # ES011). The Neutralization Dose (ND50) is typically 0.7 µg/mL. |
| 来源 | Monoclonal Rat IgG1 Clone # 369910 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11202 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Kallikrein 8/Neuropsin Gln29-Gly260 (predicted) Accession # O60259 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Kallikrein 8 (KLK8), also known as neuropsin or ovasin, is a member of the human tissue kallikrein family (1). Two alternatively spliced forms exist, resulting in 260 (isoform 1) and 305 (isoform 2) amino acid (aa) sequences, respectively (2). Isoform 1 consists of a signal peptide (residues 1 to 28), a short pro peptide (aa 29‑32) and the mature chain (aa 33-260). Isoform 2 is identical to isoform 1, except that a 45 aa segment is inserted in isoform 2 between aa 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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