Human MMP-1 MAb (Clone 36665) (25 UG)【科瑞奥博】

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Human MMP-1 MAb (Clone 36665) (25 UG)

货号： MAB901-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB901-SP
别名	CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC 3.4.24.7; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP-1
全称	Matrix Metalloproteinase 1
反应种属	Human
应用	Western Blot(2 µg/mL) Immunohistochemistry(8-25 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects pro and active forms of human MMP-1. In Western blots, no cross-reactivity with recombinant human (rh) MMP‑2, rhMMP‑3, or rhMMP‑9 is observed.
使用方法	Western Blot: 2 µg/mL Immunohistochemistry: 8-25 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human MMP‑1 (10 µg/mL, Catalog # 901-MP) cleavage of Cultrex Rat Collagen I (250 µg/mL, Catalog # 3440-100-01). The Neutralization Dose (ND₅₀) is typically 200 µg/mL.
来源	Monoclonal Mouse IgG₁ Clone # 36665
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4312 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. MMP-1 and Pro-MMP-10 as potential urinary pharmacodynamic biomarkers of FGFR3-targeted therapy in patients with bladder cancer. Authors: Du X, Lin B, Wang Q, Li H, Ingalla E, Tien J, Rooney I, Ashkenazi A, Penuel E, Qing J Clin Cancer Res, 2014;20(24):6324-35. Species: Human Sample Type: Cell Lysate Application: WB Myoferlin depletion in breast cancer cells promotes mesenchymal to epithelial shape change and stalls invasion. Authors: Li R, Ackerman WE, Mihai C PLoS ONE, 2012;7(6):e39766. Species: Human Sample Type: Cell Lysates Application: WB Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold. Authors: Cavallo C, Desando G, Facchini A, Grigolo B J Biomed Mater Res A, 2010;93(1):86-95. Species: Human Sample Type: Whole Cells Application: ICC Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins. Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT Exp. Hematol., 2009;37(7):849-56. Species: Human Sample Type: Whole Cells Application: ICC Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells. Authors: Ooi YH, Oh DJ, Rhee DJ Invest. Ophthalmol. Vis. Sci., 2009;50(11):5259-65. Species: Human Sample Type: Cell Lysates Application: WB Matrix metalloproteinase activity in pediatric acute lung injury. Authors: Kong MY, Gaggar A, Li Y Int J Med Sci, 2009;6(1):9-17. Species: Human Sample Type: Endotracheal aspirate Application: WB The association of Sam68 with Vav1 contributes to tumorigenesis. Authors: Lazer G, Peer L, Schapira V, Richard S, Katzav S Cell. Signal., 2007;19(12):2479-86. Species: Human Sample Type: Cell Lysates Application: WB Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model. Authors: Naveau A, Reinald N, Fournier B Connect. Tissue Res., 2007;48(6):300-8. Species: Human Sample Type: Whole Cells Application: DB Interleukin-1beta induced activation of nuclear factor-kappab can be inhibited by novel pharmacological agents in osteoarthritis. Authors: Lauder SN, Carty SM, Carpenter CE Rheumatology (Oxford), 2007;46(5):752-8. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21. Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F Gut, 2006;55(12):1774-80. Species: Human Sample Type: Cell Lysates Application: WB c-Kit immunophenotyping and metalloproteinase expression profiles of mast cells in interstitial lung diseases. Authors: Edwards ST, Cruz AC, Donnelly S, Dazin PF, Schulman ES, Jones KD, Wolters PJ, Hoopes C, Dolganov GM, Fang KC J. Pathol., 2005;206(3):279-90. Species: Human Sample Type: Whole Tissue Application: IHC
纯化方式	Protein A or G purified from ascites
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human MMP-1 Phe20-Asn469 Accession # P03956
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, alpha ‑1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGF-BP3, IGF-BP5, pro MMP-2 and pro MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cawston, T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.

参考图片

MMP‑1 in Human Ovarian Cancer Tissue. MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MMP‑1 in Human Ovarian Array. MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human MMP‑1 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMP‑1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.