| 货号 | MAB29071-SP |
| 别名 | wnvNS3 Protease NS2b Cofactor | 全称 | West Nile Virus NS3 Protease NS2b Cofactor |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects West Nile Virus NS2b cofactor of the NS3 Protease complex in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Viral wnvNS3 Protease (0.5 µg/mL, Catalog # 2907-SE) cleavage of the fluorogenic peptide substrate pERTKR-Amc (20 µM, Catalog # ES013). The Neutralization Dose (ND50) is typically 15 µg/mL. |
| 来源 | Monoclonal Mouse IgG3 Clone # 345712 |
| 产品组分 |
| 供应商 | R&D Systems |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. West Nile virus infection causes endocytosis of a specific subset of tight junction membrane proteins. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant wnvNS3 Protease/NS2b cofactor fusion protein Ser1423-Lys1470, Gly1506-Leu1689 Accession # ABD85079 |
| 生物活性 | Viral |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile Tris-buffered saline, pH 7.3. |
| 背景 | Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein used as an immunogen consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with a G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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