| 货号 | MAB211 |
| 别名 | LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1 | 全称 | Tumor Necrosis Factor beta |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human Lymphotoxin‑ alpha /TNF‑ beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TNF-alpha is observed. Neutralizes the biological activity of recombinant human TNF-beta. Will not neutralize the biological activity of recombinant human TNF-alpha or recombinant mouse TNF-alpha. |
| 使用方法 | Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize Lymphotoxin‑ alpha /TNF‑ beta -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.1-0.2 µg/mL in the presence of 0.25 ng/mL Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta and 1 µg/mL actinomycin D. |
| 来源 | Monoclonal Mouse IgG1 Clone # 5802 |
| 产品组分 | |
| 是否允许进口 | 否 |
| 是否危险品 | 是 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4049 (Human); 16992 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin alpha? | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant human Lymphotoxin‑ alpha /TNF‑ beta Leu35-Leu205 Accession # P01374 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Tumor necrosis factor beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF-beta /LT-alpha and TNF-alpha share approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alpha can exist as a type II membrane protein, TNF-beta /LT-alpha possesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF-beta /LT-alpha is also present on the cell surface of activated T, B and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF-beta /LT-alpha, and LT-beta are closely linked within the major histocompatibility complex. TNF-beta /LT-alpha is expressed in activated T- and B-lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alpha has been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alpha and TNF-alpha are mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alpha has also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cytotoxicity Induced by Lymphotoxin‑ alpha /TNF‑ beta and Neutralization by Human Lymphotoxin‑ alpha /TNF‑ beta Antibody. Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta (Catalog # 211‑TB) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Lymphotoxin‑ alpha /TNF‑ beta Monoclonal Antibody (Catalog # MAB211). The ND50 is typically 0.1‑0.2 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL). | |
Lymphotoxin‑ alpha /TNF‑ beta in Human PBMCs. Lymphotoxin‑ alpha /TNF‑ beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human Lymphotoxin‑ alpha /TNF‑ beta Monoclonal Antibody (Catalog # MAB211) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
