Human Lymphotoxin (a2b1/a1b2) MAb (Clone 135125) (25 UG)【科瑞奥博】

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Human Lymphotoxin (a2b1/a1b2) MAb (Clone 135125) (25 UG)

货号： MAB1370-SP 基本售价：询价规格： 25 ug

产品信息

概述
货号	MAB1370-SP
别名	Lymphotoxin
反应种属	Human
应用	Neutralization
目标/特异性	Recognizes human Lymphotoxin alpha chains in alpha ₂beta₁ or alpha ₁beta₂ trimers and also human TNF-beta. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhLIGHT, rhGITR Ligand, rhOX40 Ligand, recombinant mouse RANKL, rhTNF-alpha, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
使用方法	Neutralization: Measured by its ability to neutralize Lymphotoxin alpha 2/ beta 1-induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 0.15-0.9 µg/mL in the presence of 10 ng/mL Recombinant Human Lymphotoxin alpha 2/ beta 1 and 1 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG₁ Clone # 135125
产品组分
是否允许进口	否
是否危险品	是

性能
供应商	R&D Systems
Entrez Gene IDs	4049 (Human); 16992 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A Nat Immunol, 2014;15(4):354-64. Species: Human Sample Type: Whole Cells Application: Neut A role for lymphotoxin in primary Sjogren's disease. Authors: Shen L, Suresh L, Wu J, Xuan J, Li H, Zhang C, Pankewycz O, Ambrus JL J. Immunol., 2010;185(10):6355-63. Species: Human Sample Type: Saliva Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin alpha / beta
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Lymphotoxin alpha, also known as tumor necrosis factor (TNF) beta, and Lymphotoxin beta are members of the TNF superfamily and have been designated TNFSF1 and TNFSF3, respectively. Secreted Lymphotoxin alpha forms a homotrimer (TNF-beta) and heterotrimeric complexes with the type II membrane protein, Lymphotoxin beta (either alpha ₂beta₁ or alpha ₁beta₂ timers). These trimers serve as ligands for Lymphotoxin beta receptor, TNF RI and TNF RII and play a role in the development and maturation of the immune system.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokines and Receptors in Allergy and Asthma LTi Cells TNF Superfamily TNF Superfamily Ligands

参考图片

Cytotoxicity Induced by Lymphotoxin alpha 2/ beta 1 and Neutralization by Human Lymphotoxin Antibody.

Recombinant Human Lymphotoxin alpha ₂/ beta ₁(Catalog #679-TX) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Human Lymphotoxin alpha ₂/ beta ₁ is neutralized (green line) by increasing concentrations of Mouse Anti-Human Lymphotoxin Monoclonal Antibody (Catalog # MAB1370). The ND₅₀ is typically 0.15‑0.9 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).